Enumerating fluorescently labelled cells in a hemocytometer

A.Ferszt aferszt at ic.ac.uk
Wed Mar 3 21:31:55 EST 1999

Rudi Tapper wrote:
> Does anyone know where I can obtain "fluorescent tempera paint" ? The book
> Staining Procedures [Clark, G. (1981) 4th ed. Williams and Wilkins, page 88]
> states that
> "Since grid lines of a hemacytometer are normally not visible, the problem
> is overcome by inserting a thin piece of cardboard coated with fluorescent
> tempera paint under the swing out lens of the substage condenser. By
> manipulating the light source and condenser height, the light transmitted
> upward from the card illuminates the grid lines"
> If anyone has experience of this procedure and / or knows where I can obtain
> the paint, please could they e-mail me at Rudi.Tapper at port.ac.uk in addition
> to replying to the post on the newsgroup.
> Thanking you in advance
> Rudi
> Dr Rudi Tapper [ www.sci.port.ac.uk/ec/rudi.html ]
> Microbiology Research lab [ www.sci.port.ac.uk/~rzt/home.html ]
> University of Portsmouth
> UK

I don't know about fluorescent tempera but fluorescent fabric paints are
available in any large craft shop. Try the London Graphic Centre 0171
240 0095. They seem to stock one of everything!

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