Dear Pandora,
We routinely freeze down Hfr in 7% DMSO in TSoy Broth and freeze the
cultures at -75oC.
We haven't had any problems with it and we have been doing a lot of work
with it and MS2 phage.
Hope this helps,
Andrew Scott
Molecular Biologist
====================================================
GAP EnviroMicrobial Services Inc.
1020 Hargrieve Road
London, Ontario CANADA N6E 1P5
enviromic at execulink.com
www.execulink.com\~enviromic
====================================================
BSU wrote in message ...
>I maintain the culture collection at a local college, and I’m in the
process
>of trying to preserve bacterial specimens in 20% glycerol at –80C. While I
’
>ve been successful with most of the cultures, I’ve heard that E. coli Hfr
>specimens tend to lose their plasmids at temperatures this low. Is this
>true? And if so, is there another medium I could use to preserve this
>organism? (I don’t have access to a freeze dryer.) Any input would be
>great!
>>Thanks in advance—
>>Pandora
>>>
