Freezing E. coli Hfr

GAP EnviroMicrobial Services enviromic at execulink.com
Wed Jul 28 08:50:23 EST 1999

Dear Pandora,

We routinely freeze down Hfr in 7% DMSO in TSoy Broth and freeze the
cultures at -75oC.

We haven't had any problems with it and we have been doing a lot of work
with it and MS2 phage.

Hope this helps,

Andrew Scott
Molecular Biologist
GAP EnviroMicrobial Services Inc.
1020 Hargrieve Road
London, Ontario CANADA N6E 1P5

enviromic at execulink.com
BSU wrote in message ...
>I maintain the culture collection at a local college, and I’m in the
>of trying to preserve bacterial specimens in 20% glycerol at  –80C. While I
>ve been successful with most of the cultures, I’ve heard that E. coli Hfr
>specimens tend to lose their plasmids at temperatures this low.  Is this
>true? And if so, is there another medium I could use to preserve this
>organism? (I don’t have access to a freeze dryer.)  Any input would be
>Thanks in advance—

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