Endogenous phosphatase

Paula Murphy paulam at cs.mun.ca
Wed Jul 21 18:20:36 EST 1999

I am doing some plaque lifts and subsequently washing a nitrocellulose
membrane in 0.5%milk-TBS buffer and secondary antibody conjugated with
alkaline phosphatase. The method that I use to detect the bound secondary
antibody also detects the endogenous phosphatase. If I add organic
phosphate to the medium and wash the membranes with PBS instead of TBS
will the production of alkaline phosphatase decrease?(My results are
obtained on xray film)

paulam at ganymede.cs.mun.ca


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