I am doing some plaque lifts and subsequently washing a nitrocellulose
membrane in 0.5%milk-TBS buffer and secondary antibody conjugated with
alkaline phosphatase. The method that I use to detect the bound secondary
antibody also detects the endogenous phosphatase. If I add organic
phosphate to the medium and wash the membranes with PBS instead of TBS
will the production of alkaline phosphatase decrease?(My results are
obtained on xray film)
--Thanks,
paulam at ganymede.cs.mun.ca
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