Liz Middleton liz.middleton at lewis-associates.co.uk
Mon Jul 19 08:47:11 EST 1999

Further expanding its range of media for environmental testing, IDG has
introduced a new Buffered Charcoal Yeast extract Agar (BCYE).  Fully
conforming to BSI 6068 and ISO recommendations, this latest addition to the
LAB M catalogue includes a GVPC selective supplement and offers excellent
recovery of Legionella spp.

Current standards specify BCYE as the base agar for Legionella culture, with
the addition of GVPC supplement (Glycine, Vancomycin, Polymixin and
Cycloheximide) for efficient selectivity. The medium is recommended for the
isolation of Legionella organisms in all kinds of environmental samples
including potable, industrial and natural waters, and associated materials
such as sediments, deposits and slime.

Typically a slow growing organism, Legionella may take up to 10 days to
produce visible colonies. Subculture onto BCYE and BCYE without L-cysteine
offers the first provisional identification, with Legionella failing to grow
in the absence of L-cysteine. Alternatively, nutrient agar or blood agar may
be used in place of BCYE without
L- cysteine.
- ends ­



Editor: For more information, please contact: Phil Smith, IDG (UK) Ltd,
Topley House, 52 Wash Lane, Bury, Lancs, BL9 6AU
Phone: +44 (0) 161 797 5729, Fax: +44 (0) 161 762 9322 Email:
pasmith at idgplc.com

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