MIC Help

lamb L.A.M.Buisman at cable.A2000.nl
Sun Jan 31 11:16:45 EST 1999

What it's all about is that the final concentration of the broth is 1x.
I understand from your original question that you pipet in the wells one
part of antibiotic solution and one part of  inoculum.
So, if your inoculum is diluted in water, yes, in that case the broth
should be double strenght.
In most laboratories single strenght broth is always available, and
double strength has to be made especially, just for one time use.
That is why the most common procedure is to make all dilutions, of
antibiotic and inoculum in single strength broth.

Good luck

John Bongiovanni wrote:

>  the inoculum will be diluted in water, the antibiotic will be diluted
> in broth.  So you are saying that the antibiotic is to be made double
> strength and diluted in broth?
> lamb wrote:
>>  Just the antibiotic. As long as the dilutions are made in broth.
>> john Bongiovanni wrote:
>> >  Trying to develope new microdilution method for MIC.  Section
>> > 6.2.2 if the ANSI/NCCLS Vol. 17 states "If the inoculum is to added
>> > by pipet as described in section, the antimicrobial
>> > solutions are prepared the at twice the desired final concentration
>> > and the wells are filled with 0.05 mL instead of 0.1 mL."
>> >
>> > Q - Are both the antimicrobial substrate and broth  made double
>> > strength or is just the antimicrobial?
>> > Thanx in advance
>> >

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