MIC Help

John Bongiovanni bongi at gower.net
Sun Jan 31 00:49:58 EST 1999

the inoculum will be diluted in water, the antibiotic will be diluted in
broth.  So you are saying that the antibiotic is to be made double
strength and diluted in broth?

lamb wrote:

>  Just the antibiotic. As long as the dilutions are made in broth.
> john Bongiovanni wrote:
>>  Trying to develope new microdilution method for MIC.  Section 6.2.2
>> if the ANSI/NCCLS Vol. 17 states "If the inoculum is to added by
>> pipet as described in section, the antimicrobial solutions
>> are prepared the at twice the desired final concentration and the
>> wells are filled with 0.05 mL instead of 0.1 mL."
>> Q - Are both the antimicrobial substrate and broth  made double
>> strength or is just the antimicrobial?
>> Thanx in advance
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