Just the antibiotic. As long as the dilutions are made in broth.
john Bongiovanni wrote:
> Trying to develope new microdilution method for MIC. Section 6.2.2
> if the ANSI/NCCLS Vol. 17 states "If the inoculum is to added by pipet
> as described in section 6.3.2.2, the antimicrobial solutions are
> prepared the at twice the desired final concentration and the wells
> are filled with 0.05 mL instead of 0.1 mL."
>> Q - Are both the antimicrobial substrate and broth made double
> strength or is just the antimicrobial?
> Thanx in advance
>
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