MIC Help

lamb L.A.M.Buisman at cable.A2000.nl
Sat Jan 30 14:18:22 EST 1999

Just the antibiotic. As long as the dilutions are made in broth.

john Bongiovanni wrote:

>  Trying to develope new microdilution method for MIC.  Section 6.2.2
> if the ANSI/NCCLS Vol. 17 states "If the inoculum is to added by pipet
> as described in section, the antimicrobial solutions are
> prepared the at twice the desired final concentration and the wells
> are filled with 0.05 mL instead of 0.1 mL."
> Q - Are both the antimicrobial substrate and broth  made double
> strength or is just the antimicrobial?
> Thanx in advance

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