In article <evrendilek.1.8.36A7BDAD at osu.edu>,
evrendilek.1 at osu.edu (Fatih Evrendilek) wrote:
> Hi,
> After a processing to inactivatea specific bacterial population (e.g. S.
> aureus) in any kind of liquid media, how can you seperate dead and living
> cells?
> Assume that the treatment destroys the cell membrane. In addition, in
> your liquid media not every bacterial cell is not treated equally meaning your
> treatment is not homogenous throughout the system thus. you have not injured,
> injured, and death cells. In this case some cell organells will go out liquid
> media from the injured and death cells and the density of that cells would be
> different than the one that has not been destroyed. But I am not sure if
> density difference will help me?
> Thanks
> Gulsun
Your method of choice are fluorescent probes. You can actually detect the
viability of antibody labeled cells in a mixture of dental plaque (see
http://www.cyto.purdue.edu/flowcyt/research/micrflow/gerhard/gerhard.htm ).
However, you have to consider the mode of action of your treatment. If you
slice the DNA for example you can not detect cell death by membrane integrity
or enzyme activity measurements. The other pittfall is that most cells dye as
a consequence of your attempt to grow them after injury. For optimum recovery
you might want to contact Oxoid (www.oxoid.co.uk) and enquire about the
SPRINT medium.
Good luck
Gerhard Nebe-v.Caron
Unilever Research, Colworth,
Sharnbrook, Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222000
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