distinguishing living and dead cells

lamb L.A.M.Buisman at cable.A2000.nl
Fri Jan 22 14:58:36 EST 1999

Don't forget that de population before treatment also contains dead cells.
Do you want to separate the dead from the living or determine how effective the
treatment is?
Effectiveness can be measured by a viable count (plating of dilutions) before and
after treating.

Fatih Evrendilek wrote:

>         Hi,
>         After a processing to inactivatea specific bacterial population (e.g. S.
> aureus)  in any kind of liquid media, how can you seperate dead and living
> cells?
>         Assume that the treatment destroys the cell membrane. In addition, in
> your liquid media not every bacterial cell is not treated equally meaning your
> treatment is not homogenous throughout the system thus. you have not injured,
> injured, and death cells. In this case some cell organells will go out liquid
> media from the injured and death cells and the density of that cells would be
> different than the one that has not been destroyed. But I am not sure if
> density difference will help me?
>         Thanks
>                 Gulsun

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