At 11:52 PM 1/21/99 GMT, Fatih Evrendilek wrote:
> Hi,
> After a processing to inactivatea specific bacterial population (e.g. S.
>aureus) in any kind of liquid media, how can you seperate dead and living
>cells?
> Assume that the treatment destroys the cell membrane. In addition, in
>your liquid media not every bacterial cell is not treated equally meaning your
>treatment is not homogenous throughout the system thus. you have not injured,
>injured, and death cells. In this case some cell organells will go out liquid
>media from the injured and death cells and the density of that cells would be
>different than the one that has not been destroyed. But I am not sure if
>density difference will help me?
> Thanks
> Gulsun
>>
I am at all sure I understand your statements and questions. AFAIK, you can
not separate dead from living cells from a liquid medium, since all cells...
dead or alive tend to have very similar density. Was that your query?
However, you can do "viable counts" from a liquid culture by plating known
dilutions of the same on an appropriate plate.
Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu
