IUBio

distinguishing living and dead cells

Fatih Evrendilek evrendilek.1 at osu.edu
Thu Jan 21 18:52:13 EST 1999


	Hi,
	After a processing to inactivatea specific bacterial population (e.g. S. 
aureus)  in any kind of liquid media, how can you seperate dead and living 
cells?	
	Assume that the treatment destroys the cell membrane. In addition, in 
your liquid media not every bacterial cell is not treated equally meaning your 
treatment is not homogenous throughout the system thus. you have not injured, 
injured, and death cells. In this case some cell organells will go out liquid 
media from the injured and death cells and the density of that cells would be 
different than the one that has not been destroyed. But I am not sure if 
density difference will help me?
	Thanks
		Gulsun 



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