Vibrio alginolyticus is fairly undemanding in its nutritional
requirements. We use this species quite a lot at our department, and find
that a very simple marine medium works well:
PER LITRE:
750mL seawater (32ppt)
250mL distilled water
5g peptone
3g yeast extract
15g agar (omit if liquid broth required).
I've just pulled out some very dense cultures of alginolyticus grown with
this medium, so you can be assured it works ! The medium is obviously not
selective for Vibrio's.
John Rowlands
Senior Technician (Genetics & Biotechnology Group)
School of Ocean Sciences,
University of Wales, Bangor.
