Dear All,
This is aimed at those molecular biologists working with these bacteria. I
am having difficulty amplifying a gene identified from the whole genome
project.
Are there any tricks to PCR amplifying from these bacteria? Such as DMSO
etc.(I had no joy with a 6percent solution).
I am trying to amplify from cell pellets. Has anyone had success
amplifying from bacterial pellets? I heat to 95 degrees C for 5 mins prior
to the PCR cycle.
Does anyone have a cDNA library perhaps from B.subtilis that I could have
an aliquot of to try to PCR from this?
I have contacted the people involved in the whole genome project and they
do not have the relevant DNA to distribute as a shot gun sequencing
strategy was employed. Bit of a downfall with the whole genome project
perhaps.
I would be extremly grateful for any help.
Much thanks,
Mark
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Dr.M.Payton,
Department of Pharmacology,
University of Oxford,
Oxford.
OX1 3QT
U.K.
Tel; +441865 271595
fax; +441865 271853
email; mpayton at worf.molbiol.ox.ac.uk
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