Review Article: PCR and Clinical Applications

stewartl at alkami.com stewartl at alkami.com
Thu Jan 7 18:14:33 EST 1999

"Dot blots and reverse dot blots are techniques where PCR products are
hybridized with allele specific oligonucleotides (ASOs) for the purpose
of scanning for mutations, detecting RNA, cancer, or pathogens". 

The full text Alkami PCR Review of "Hybridizing with a reason: Clinical
Applications of Dot Blots " by Dr. Laura Ruth is now available at

The Alkami Biosystems web site is devoted to offering FREE resources and
information related to the polymerase chain reaction (PCR). The site
includes resources for primer design, PCR methods, troubleshooting,
polymerases, and other PCR related topics.

Order a FREE PCR Tube sample pack at http://www.alkami.com/pgorder.htm

Primer Design Tips - http://www.alkami.com/refprmr.htm
Primer design advice collected from a wide range of sources.

Primer Design Online - http://www.alkami.com/refdsgn.htm
Links to free online primer design tools with brief program descriptions
of each one.

PCR Troubleshooting - http://www.alkami.com/reftrbl.htm
Tips for improving your PCR specificity and efficiency, enhancers and
inhibitors, what to do for common PCR problems, and more.

PCR Methods - http://www.alkami.com/refmthd.htm
PCR method descriptions (such as RT-PCR, Hot Start PCR, In Situ, etc),
sample protocols, and related reference links.

Polymerases - http://www.alkami.com/refpoly.htm
Descriptions of commonly used thermophilic polymerases including a short
overview of characteristics (such as error rates), suggested ranges for
selected variables, and relevant reference links.

PCR Variables - http://www.alkami.com/refvrbls.htm
PCR experiment variables each with standard ranges, general information,
and ways to improve specificity, efficiency, and/or fidelity.

Visit our site at http://www.alkami.com and let us know if you
have any questions or suggestions about how we can make the Alkami
Biosystems web site an even better PCR resource for you and your

Simplified Solutions for Complex Questions tm
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