Separate your chromosomal fraction into three tubes. Add RNase to one of
the tubes, DNase to another, and leave the other tube as a control. Run
aliquots of all three tubes (post-incubation) in separate lanes. You
should be able to distinguish which bands are which.
Abdus Samad Cassim wrote:
> Hi .
>> Upon the isolation of Chromosomal DNA , plasmid D.N.A and R.N.A , we
> obtained a photograph of the gel ( in a gel documentation system ) .
> However , I had a specific problem . How is it possible to prove
> and Distingiush which bands are the D.N.A and which are the R.N.A
>> Thanks in Advance.
>> Abdus Samad
> Rhodes University
> South Africa
> Dept Micro + Biochem