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Reducing agents for protein chemistry

Achim Recktenwald, PhD ARecktenwald at StressGen.com
Fri Aug 13 13:00:16 EST 1999

Hi everybody,

For some time already, I am looking for a paper / book / webpage / or any
other source that contains a list of redox potentials for commonly used
reducing agents, like:

beta-mercaptoethanol (BME), dithiothreitol (DTT), glutathione, cysteine,
Tris(2-carboxyethyl)phosphine hydrochloride (TCEP.HCl) and so on. There must
be others around, as well.

In many references you can find that DTT is about 10fold better than BME.
But I found the wording always so similar that I assume the authors are just
copying from each other.
Does anybody know of a more exact reference, possibly even including more

We have to produce proteins for in vivo experiments and cannot use BME or
DTT in final preparations, because of their toxicity. In addition, I would
be interested in their relative strength, i.e., their redox potential.

Any answer would be appreciated If possible, send me a copy of your answer
also by email



ARecktenwald at StressGen.com

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