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Inclusion bodies

Achim Recktenwald achimr at home.com
Wed Aug 4 13:43:22 EST 1999


Chris Fields <cjfields at jove.acs.unt.edu> wrote in message
news:37A5C2A3.BD917557 at jove.acs.unt.edu...
>
>
> Dr Lester Davids wrote:
> >
> > Hi All,
> >
> > Looking for advice as to how to PREVENT proteins from getting into these
> > damn bacterial inclusion bodies! It seems out native proteins are not
> > but our mutant proteins are. Any suggestions out there?
> >
> > Thanx,
> > Lester Davids
>
> Try growing at different temperatures (we had luck at 25-30 degrees)
[snip]


After growing E.coli at 35 - 37C, I've went as low as 21C for the expression
of a recombinant protein to avoid IBs.

Achim






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