Dr Lester Davids wrote:
>> Hi All,
>> Looking for advice as to how to PREVENT proteins from getting into these
> damn bacterial inclusion bodies! It seems out native proteins are not
> but our mutant proteins are. Any suggestions out there?
> Lester Davids
Try growing at different temperatures (we had luck at 25-30 degrees).
Also, I have read something related to this on the bionet.proteins group
about coexpressing a chaparone protein for folding. You may also want
to consider denaturation-renaturation using urea or guanidium
C. J. Fields
Dept. of Biological Sciences
The University of North Texas