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Registration for the Post Doctoral Position

Bandivadekar Rajiv RAJIV at koel.co.in
Sun Aug 1 14:37:24 EST 1999


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I understand that you provide Job Opportunities to those aspiring for Post
Doctoral Positions.

I am a Doctorate in Microbiology and currently working as a Lecturer in the
Department of Microbiology, University of Pune, India 411 007. During my
Doctoral Research, I have worked on enzyme xylanase from an actinomyces sp.,
which involved work in the areas of Protein Chemistry and Gene Cloning. I am
appending herewith an abstract of my thesis along-with my Curriculum Vitae,
in the MS Word 6.0/95 File :

  <<CV_Std_Resume.doc>> 
I wish to get my name and CD registered on your Web Site for the
Post-Doctoral Position, preferably in countries like USA, UK and Canada in
that order. My areas of interest are in the fields of Molecular Biology and
Protein Engineering of microbial systems. You may forward my E_Mail to the
prospecting Organisations, under copy to me.

Looking forward to hearing from you, soon on E_Mail ID rajiv at koel.co.in
<mailto:rajiv at koel.co.in> .
      
Best Regards


Kavita Bandivadekar : Camp ==> Home
Respond To ==> rajiv at koel.co.in <mailto:rajiv at koel.co.in>  



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\'b7}}\ls15\adjustright {\f15\fs22\cgrid0 Place of birth\tab : \tab =
Akola
\par }\pard \li2160\nowidctlpar{\*\pn =
\pnlvlcont\ilvl0\ls0\pnrnot0\pndec }\adjustright {\f15\fs6\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls15\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls15\adjustright {\f15\fs22\cgrid0 Nationality\tab : \tab Indian
\par }\pard \li2160\nowidctlpar{\*\pn =
\pnlvlcont\ilvl0\ls0\pnrnot0\pndec }\adjustright {\f15\fs6\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls15\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls15\adjustright {\f15\fs22\cgrid0 Caste\tab \tab : \tab Brahmin
\par }\pard \li2160\nowidctlpar{\*\pn =
\pnlvlcont\ilvl0\ls0\pnrnot0\pndec }\adjustright {\f15\fs6\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls15\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls15\adjustright {\f15\fs22\cgrid0 Address \tab \tab : \tab 3, =
Bhagyashri, Rambag Colony,\tab=20
\par }\pard \li2520\nowidctlpar\adjustright {\b\f15\cgrid0 (Permanent =
as well}{\f15\cgrid0  \tab    \tab }{\f15\fs22\cgrid0 Paud =
Road,}{\f15\cgrid0 \tab=20
\par }{\b\f15\cgrid0 as Contact)\tab }{\f15\cgrid0 \tab \tab PUNE 411 =
038
\par }\pard \li1800\nowidctlpar\adjustright {\f15\fs16\cgrid0=20
\par }\pard\plain \s23\qj\li2160\nowidctlpar\adjustright \f15\fs20 =
{\fs22 Awarded coveted Research Fellowship by the Council of Scientific =
& Industrial Research, Ministry of Science & Technology, Government of =
India, for 5 years.=20
\par }\pard\plain \li1800\nowidctlpar\adjustright \fs20\cgrid =
{\f15\cgrid0=20
\par {\pntext\pard\plain\f14\lang1033 \loch\af14\dbch\af0\hich\f14 =
\'d8\tab}}\pard \fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls30\pnrnot0\pnf14\pnfs24\pnstart1\pnindent360\pnhang{\p=
ntxtb \'d8}}\ls30\adjustright {\b\f15\fs22\cgrid0 About Self :
\par }\pard \li1800\nowidctlpar\adjustright {\f15\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls14\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls14\adjustright {\f15\fs22\cgrid0 Quiet but die hard by nature, =
eager to acquire knowledge & skills in allied fields and a keen =
observer. In pursuit of an objective, I analyse any given situation by =
scanning the same to its roots and taking the same to its logical =
conclusion.
\par }\pard \qj\li2160\nowidctlpar{\*\pn =
\pnlvlcont\ilvl0\ls0\pnrnot0\pndec }\adjustright {\f15\fs12\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls14\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls14\adjustright {\f15\fs22\cgrid0 Interested in Basic as well =
as Applied Research. Specific areas of interests are Biochemistry and =
Molecular Biology
\par }\pard \li1800\nowidctlpar\adjustright {\f15\fs16\cgrid0=20
\par {\pntext\pard\plain\f14\fs22\lang1033 \loch\af14\dbch\af0\hich\f14 =
\'31\tab}}\pard \fi-360\li2160\nowidctlpar{\*\pn =
\pnlvlblt\ilvl0\ls2\pnrnot0\pnf14\pnfs22\pnstart1\pnindent360\pnhang{\pn=
txtb 1}}\ls2\adjustright {\b\f15\fs22\ul\cgrid0 =
EDUCATION}{\f15\fs22\cgrid0=20
\par }\pard \fi-1800\li1800\nowidctlpar\adjustright {\i\f15\fs16\cgrid0 =

\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls16\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls16\adjustright {\b\f15\fs22\cgrid0 1978,}{\f15\fs22\cgrid0  =
Standard X, Maharashtra State Board of Secondary & Higher Secondary =
Education, % Marks 77.8%, Distinction Grade
\par }\pard \qj\li2160\nowidctlpar\adjustright {\f15\fs12\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls21\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls21\adjustright {\b\f15\fs22\cgrid0 1980,}{\f15\fs22\cgrid0  =
Standard XII, Maharashtra State Board of Secondary & Higher Secondary =
Education, % Marks 65.5%, First Class\tab=20
\par }\pard \qj\li2160\nowidctlpar\adjustright {\f15\fs12\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls22\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls22\adjustright {\b\f15\fs22\cgrid0 1983,}{\f15\fs22\cgrid0  =
B.Sc. (Microbiology), Pune University, % Marks 68.2 %, First Class
\par }\pard \qj\li2160\nowidctlpar\adjustright {\f15\fs12\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls19\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls19\adjustright {\b\f15\fs22\cgrid0 1985,}{\f15\fs22\cgrid0  =
M.Sc. (Applied Microbiology), Pune University, Marks 60%, First Class
\par }\pard \qj\li2160\nowidctlpar\adjustright {\f15\fs12\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls20\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls20\adjustright {\b\f15\fs22\cgrid0 1999,}{\f15\fs22\cgrid0  =
Ph.D. (Microbiology), Pune University
\par }\pard \li1800\nowidctlpar\adjustright {\f15\fs12\cgrid0=20
\par }{\b\f15\fs22\cgrid0 Thesis Title \ldblquote Molecular and =
Biochemical aspects of }{\b\i\f15\fs22\cgrid0 =
Chainia}{\b\f15\fs22\cgrid0  Sp.\rdblquote  }{\b\i\f15\fs22\cgrid0 =
(Refer Enclosure I for Thesis Abstract and List of Publications)
\par }{\f15\fs12\cgrid0=20
\par=20
\par=20
\par=20
\par=20
\par=20
\par }{\b\f15\fs22\cgrid0 Research Guide :=20
\par }{\f15\fs12\cgrid0=20
\par }{\f15\fs22\cgrid0 Dr. (Mrs) Vasanti Deshpande, Scientist F, =
National Chemical Laboratory, Pashan, PUNE 411 008.
\par }{\f15\fs16\cgrid0=20
\par {\pntext\pard\plain\f14\fs22\lang1033 \loch\af14\dbch\af0\hich\f14 =
\'31\tab}}\pard \fi-360\li2160\nowidctlpar{\*\pn =
\pnlvlblt\ilvl0\ls2\pnrnot0\pnf14\pnfs22\pnstart1\pnindent360\pnhang{\pn=
txtb 1}}\ls2\adjustright {\b\f15\fs22\ul\cgrid0 EMPLOYMENT
\par }\pard \fi-1800\li1800\nowidctlpar\adjustright {\i\f15\fs16\cgrid0 =

\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls32\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls32\adjustright {\f15\fs22\cgrid0 Lecturer in the Department of =
Microbiology, University of Pune,         411 007, India, since June =
99. Total Emoluments Rs 10,000 p.m.
\par }\pard \qj\li2160\nowidctlpar\adjustright {\f15\fs22\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls25\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls25\outlinelevel0\adjustright {\f15\fs22\cgrid0 Lab In-charge, =
Water & Waste Water Research Center, Pune, Jan 1986-June 1987, Total =
Emoluments Rs. 1000/- p.m.
\par }\pard \qj\li2160\nowidctlpar\adjustright {\f15\fs12\cgrid0=20
\par }\pard \qj\li2520\nowidctlpar\adjustright {\f15\fs22\cgrid0 =
Analysis Industrial Effluents with special emphasis on harmful =
chemicals, BOD and COD. Independent handling of assignment
\par }\pard\plain \s24\qj\li2160\nowidctlpar\outlinelevel0\adjustright =
\f15\fs20 {\fs22=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard\plain =
\qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls24\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls24\outlinelevel0\adjustright \fs20\cgrid {\f15\fs22\cgrid0 =
Medical Representative, Pharma Remedies, Mumbai, October -December =
1986, Total Emoluments Rs. 1500/- p.m. approx.
\par }\pard \qj\li2160\nowidctlpar\adjustright {\f15\fs12\cgrid0=20
\par }\pard \qj\li2520\nowidctlpar\adjustright {\f15\fs22\cgrid0 =
Selling Company Products through visits to Doctors and Chemists. =
Experiences gathered viz.; Product Promotion, Handling of =
Queries/Objections etc.
\par }\pard\plain \s24\qj\li2160\nowidctlpar\outlinelevel0\adjustright =
\f15\fs20 {\fs22=20
\par {\pntext\pard\plain\s24 \f3\fs22\lang1033 =
\loch\af3\dbch\af0\hich\f3 \'b7\tab}}\pard =
\s24\qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls23\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls23\outlinelevel0\adjustright {\fs22 Lab Technician, Poona =
Hospital & Research Center, Pune, June-September 1985, Total Emoluments =
Rs. 650/-  p.m.
\par }\pard\plain \qj\li2160\nowidctlpar\adjustright \fs20\cgrid =
{\f15\fs12\cgrid0=20
\par }\pard \qj\li2520\nowidctlpar\outlinelevel0\adjustright =
{\f15\fs22\cgrid0 Analysis of Pathological Samples. Exposure to Lab =
Environment.=20
\par }\pard \qj\li2160\nowidctlpar\adjustright {\f15\fs12\cgrid0=20
\par=20
\par }\pard \qj\li2520\nowidctlpar\adjustright {\f15\fs22\cgrid0=20
\par }\pard \li1800\nowidctlpar\adjustright {\f15\fs16\cgrid0=20
\par {\pntext\pard\plain\f14\fs22\lang1033 \loch\af14\dbch\af0\hich\f14 =
\'31\tab}}\pard \fi-360\li2160\nowidctlpar{\*\pn =
\pnlvlblt\ilvl0\ls2\pnrnot0\pnf14\pnfs22\pnstart1\pnindent360\pnhang{\pn=
txtb 1}}\ls2\adjustright {\b\f15\fs22\ul\cgrid0 INTERESTS & ACTIVITIES
\par }\pard \li1800\nowidctlpar\adjustright {\f15\fs16\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls26\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls26\adjustright {\f15\fs22\cgrid0 Keen interest in Nature =
Conservation with focus on Birds. Have volunteered in activities like =
participation in Bird Census, organising Nature Camps.=20
\par }\pard \qj\li2160\nowidctlpar{\*\pn =
\pnlvlcont\ilvl0\ls0\pnrnot0\pndec }\adjustright {\f15\fs12\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls26\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls26\adjustright {\f15\fs22\cgrid0 State Level Athlete (Events =
like Javelin Throw, Discus Throw, and Shot put).
\par }\pard \li1800\nowidctlpar\adjustright {\f15\fs16\cgrid0=20
\par {\pntext\pard\plain\f14\fs22\lang1033 \loch\af14\dbch\af0\hich\f14 =
\'31\tab}}\pard \fi-360\li2160\nowidctlpar{\*\pn =
\pnlvlblt\ilvl0\ls2\pnrnot0\pnf14\pnfs22\pnstart1\pnindent360\pnhang{\pn=
txtb 1}}\ls2\adjustright {\b\f15\fs22\ul\cgrid0 COMPUTER SKILLS
\par }\pard \li1800\nowidctlpar\adjustright {\f15\fs16\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls27\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls27\adjustright {\f15\fs22\cgrid0 Familiar with software\rquote =
s like Microsoft Office 97, Microsoft Outlook 97, DNAsis
\par }\pard \qj\li2160\nowidctlpar{\*\pn =
\pnlvlcont\ilvl0\ls0\pnrnot0\pndec }\adjustright {\f15\fs12\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls27\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls27\adjustright {\f15\fs22\cgrid0 Familiar with Internet =
Surfing
\par }\pard \li1800\nowidctlpar\adjustright {\f15\fs16\cgrid0=20
\par {\pntext\pard\plain\f14\fs22\lang1033 \loch\af14\dbch\af0\hich\f14 =
\'31\tab}}\pard \fi-360\li2160\nowidctlpar{\*\pn =
\pnlvlblt\ilvl0\ls2\pnrnot0\pnf14\pnfs22\pnstart1\pnindent360\pnhang{\pn=
txtb 1}}\ls2\adjustright {\b\f15\fs22\ul\cgrid0 PROJECT EXPERIENCE
\par }\pard \li1800\nowidctlpar\adjustright {\f15\fs16\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls29\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls29\adjustright {\f15\fs22\cgrid0 Six Months Field and Lab =
Research as a part of M.Sc. Curriculum on an Industrial Project titled, =
\ldblquote Isolation of Cyanide Degrading Organisms with particular =
reference to bacteria\rdblquote .
\par }\pard \qj\nowidctlpar{\*\pn \pnlvlcont\ilvl0\ls0\pnrnot0\pndec =
}\adjustright {\f15\fs12\cgrid0=20
\par {\pntext\pard\plain\f3\fs22\lang1033 \loch\af3\dbch\af0\hich\f3 =
\'b7\tab}}\pard \qj\fi-360\li2520\nowidctlpar\jclisttab\tx2520{\*\pn =
\pnlvlblt\ilvl0\ls29\pnrnot0\pnf3\pnstart1\pnindent360\pnhang{\pntxtb =
\'b7}}\ls29\adjustright {\f15\fs22\cgrid0 Six Months Field and Lab =
Research, as a part of Project by Ecological Society of India. Project =
was titled \ldblquote Microbial Analysis of Soil & Water around Warvand =
Lake\rdblquote , near Pune.
\par }\pard\plain \s17\qc\sa120\nowidctlpar\adjustright \fs20\lang2057 =
{\b\fs32\ul=20
\par }\pard \s17\qc\sa120\nowidctlpar\outlinelevel0\adjustright =
{\b\fs32\ul ENCLOSURE I
\par {\pntext\pard\plain\s17 \b\f14\fs28\cf1 =
\loch\af14\dbch\af0\hich\f14 \'d8\tab}}\pard =
\s17\qj\fi-360\li360\sa120\nowidctlpar\jclisttab\tx360{\*\pn =
\pnlvlblt\ilvl0\ls10\pnrnot0\pnb1\pni0\pncf1\pnf14\pnfs28\pnstart1\pnind=
ent360\pnhang{\pntxtb \'d8}}\ls10\adjustright {\b\fs32 ABSTRACT :
\par }\pard \s17\qj\sa120\sl360\slmult1\nowidctlpar\adjustright =
{\f15\fs22 The current increased demand for better utilisation of =
replenishable sources and the pressure on the industries to operate =
within environmentally compatible limits has been a stimulus to the =
development of new concepts in Biotechnology. Agriculture and forestry =
waste contains polysaccharides, which could be converted to valuable =
fermentable sugars for the production of variety of chemicals like =
ethanol. Xylans or hemicelluloses are the heterologous polysaccharides =
and one of the important renewable resources found in the cell walls of =
plants. Xylan has a high potential for its degradation to useful end =
products. Xylanases produced by various organisms are useful in xylan =
degradation.}{\i\f15\fs22  Chainia}{\f15\fs22  (NCL 82.5.1) a =
sclerotial actinomycete species isolated in our laboratory produces =
many commercially important enzymes such as cellulase-free xylanase and =
substrate specific intra and extra cellular glucose/xylose isomerases. =
The ready accessibility of the enzyme to cellulose pulp due to its =
small size and the absence of cellulase are its advantageous features. =
In view of its industrial importance, it was planned to study the =
molecular and biochemical aspects of }{\i\f15\fs22 Chainia}{\f15\fs22  =
by studying structure-function relationship of the xylanase and =
molecular cloning of the gene responsible for its activity.
\par }\pard\plain \s2\qj\keepn\nowidctlpar\outlinelevel1\adjustright =
\cbpat8 \fs28\lang2057 {\b\f15\ul SUMMARY OF WORK
\par }\pard\plain \s17\qj\sa120\nowidctlpar\adjustright \fs20\lang2057 =
{\b\f15\fs24=20
\par }{\b\f15\fs36 {\field{\*\fldinst SYMBOL 38 \\f "Wingdings" \\s =
18}{\fldrslt\f14\fs36}}}{\b\f15  }{\b\f15\fs28 Structure-function =
relationship of xylanase II from }{\b\i\f15\fs28 Chainia.}{\b\f15\fs24=20
\par {\pntext\pard\plain\s4 \b\f14\fs28\cf1 =
\loch\af14\dbch\af0\hich\f14 \'32\tab}}\pard\plain =
\s4\qj\li720\sb240\sa60\sl360\slmult1\keepn\nowidctlpar\jclisttab\tx1080=
{\*\pn =
\pnlvlblt\ilvl0\ls7\pnrnot0\pnb1\pni0\pncf1\pnf14\pnfs28\pnstart1\pninde=
nt360 {\pntxtb 2}}\ls7\outlinelevel3\adjustright \b\i\lang2057 =
{\i0\f15\ul Study of microenvironment of tryptophan (trp) =
residue.}{\b0\i0\f15=20
\par }\pard\plain \s17\qj\sa120\sl360\slmult1\nowidctlpar\adjustright =
\fs20\lang2057 {\f15\fs24 Trp is a useful built-in fluorescent probe. =
Its fluorescence is very sensitive to its microenvironment. Involvement =
of one out of three trp residues in the active site of the xylanase II =
from }{\i\f15\fs24 Chainia}{\f15\fs24  has been demonstrated =
previously, [Deshpande, Hinge and Rao (1990) Biochem. Biophys. Acta =
1041, 172 - 177]. The work described here aims at : (i) deducing the =
structure-function relationship for the trp residue involved at the =
active site (a) by correlating the effect of N - bromosuccinimide (NBS) =
on the fluorescence and activity, and (b) by assessing the ability of =
xylan to protect against decrease in fluorescence versus activity of =
NBS-treated enzyme; and (ii) probing into the environment of the trp =
residues by studying the quenching of their fluorescence by=20
\par=20
\par various solute quenchers in presence and absence of guanidine =
hydrochloride (Gdn.HCl). Complete inactivation of NBS-treated enzyme =
occurs well before the loss of fluorescence. Full protection by xylan =
(0.5%), of the inactivation of enzyme by NBS compared with 30% =
protection for the decrease in fluorescence, confirms the participation =
of a single trp at substrate binding site of the xylanase. The xylanase =
exhibited a rather low fluorescence emission maximum at 310 nm. There =
was no shift in the emission maximum on treatment of the enzyme with =
Gdn.HCl (Upto 6.5 M), indicating the rigidity of the microenvironment =
around trp residues. The quenching studies with acrylamide suggested =
the occurrence of both collisional as well as static quenching =
processes. The enzyme retained full activity as well as the =
characteristic emission maximum at 310 nm in the presence of acrylamide =
(100 mM), indicating that the quenching of fluorescence by acrylamide =
is a physical process. Acrylamide was more efficient as a quencher than =
CsCl and KBr. Treatment of the enzyme with Gdn.HCl resulted in an =
increase in the Stern-Volmer quenching constants (K}{\f15\fs24\sub =
sv}{\f15\fs24 ) of the solute quenchers. The analysis of =
K}{\f15\fs24\sub sv}{\f15\fs24  and V values of KBr and CsCl suggests =
that the overall trp microenvironment in the xylanase II from =
}{\i\f15\fs24 Chainia}{\f15\fs24  is slightly electronegative.
\par {\pntext\pard\plain\s17 \b\f14\fs28\cf1 =
\loch\af14\dbch\af0\hich\f14 \'32\tab}}\pard =
\s17\qj\li720\sa120\sl360\slmult1\nowidctlpar\jclisttab\tx1080{\*\pn =
\pnlvlblt\ilvl0\ls8\pnrnot0\pnb1\pni0\pncf1\pnf14\pnfs28\pnstart1\pninde=
nt360 {\pntxtb 2}}\ls8\adjustright {\b\f15\fs24\ul Study of structural =
environment of an essential cysteine residue}{\f15\fs24=20
\par }\pard\plain \s15\qj\sa120\sl360\slmult1\nowidctlpar\adjustright =
\fs20\lang2057 {\f15\fs22 N-(2,4-Dinitroanilino) maleimide reacts =
covalently with the thiol group of the xylanase II from }{\i\f15\fs22 =
Chainia}{\f15\fs22  leading to complete inactivation in a manner =
similar to N-ethylmaleimide, but provides a reporter group at the =
active site of the enzyme. Increasing amounts of xylan offered enhanced =
protection against inactivation of the xylanase by DAM. Xylan (5 mg) =
showed complete protection, providing evidence for the presence of =
cysteine at the substrate-binding site of the enzyme. Kinetics of the =
chemical modification of the xylanase by DAM indicated the involvement =
of 1 mol of cysteine residue per mol of enzyme, as reported earlier. =
The second order rate constant for the reaction of DAM with the enzyme =
was 3.61 X 10}{\f15\fs22\super 3}{\f15\fs22  M}{\f15\fs22\super =
-1}{\f15\fs22 min}{\f15\fs22\super -1.}{\f15\fs22  The purified =
xylanase was alkylated with DAM and digested with pepsin. The peptides =
were separated by gel filtration. The specific modified cysteinyl =
peptide was further purified by reverse phase HPLC. The active site =
peptide was located visually by its predominant yellow colour and =
characterised by a higher A}{\f15\fs22\sub 340}{\f15\fs22  / =
A}{\f15\fs22\sub 210}{\f15\fs22  ratio. The modified active site =
peptide has the sequence:  Glu \endash  Thr \endash  Phe \endash  Xaa =
\endash  Asp. The sequence of the peptide was distinctly different from =
that of cysteinyl peptide derived from a xylanase from a thermotolerant =
}{\i\f15\fs22 Streptomyces}{\f15\fs22 =20
\par=20
\par species, but showed the presence of a conserved aspartic acid =
residue consistent with the catalytic regions of other glucanases. =20
\par {\pntext\pard\plain\s17 \b\f14\fs28\cf1 \loch\af14\dbch\af0\hich\f1=
4 \'32\tab}}\pard\plain =
\s17\qj\li720\sa120\sl360\slmult1\nowidctlpar\jclisttab\tx1080{\*\pn =
\pnlvlblt\ilvl0\ls9\pnrnot0\pnb1\pni0\pncf1\pnf14\pnfs28\pnstart1\pninde=
nt360 {\pntxtb 2}}\ls9\adjustright \fs20\lang2057 {\b\f15\fs24\ul The =
Evidence for essential carboxylate residue}{\b\f15 =20
\par }\pard \s17\qj\sa120\sl360\slmult1\nowidctlpar\adjustright =
{\f15\fs22 The role of carboxyl group in the catalytic mechanism of the =
xylanase from }{\i\f15\fs22 Chainia}{\f15\fs22  has been demonstrated =
for the first time, based on the specific interaction of guanidine =
hydrochloride (Gdn.HC1) and Woodwards reagent K (WRK) with the carboxy1 =
groups of the protein. The pH dependence of the kinetic parameters for =
hydrolysis of xylan implied the requirement of carboxy1 groups for the =
activity of the enzyme. Low concentrations of Gdn.HC1 inhibited the =
xylanase activity reversibly and competitively with a Ki of 0.63 M and =
the inhibition was unaccompanied by the structural changes in the =
protein. WRK completely inactivated the xylanase with the second order =
rate constant of 144 M}{\f15\fs22\super -1}{\f15\fs22 =
min}{\f15\fs22\super -1}{\f15\fs22 . Xylan afforded complete protection =
against inactivation by WRK, indicating the involvement of carboxylate =
group at the substrate binding region of the enzyme. Earlier =
observation of the presence of a single aspartate residue (Asp =
}{\f15\fs22\super 21}{\f15\fs22 ) in the conserved catalytic region =
together with the present evidence for the essential carboxylate =
residue in the active site suggests that Asp}{\f15\fs22\super 21  =
}{\f15\fs22 is involved in the catalysis of xylan hydrolysis by =
}{\i\f15\fs22 Chainia}{\f15\fs22  xylanase.
\par }{\b\f15\fs36 {\field{\*\fldinst SYMBOL 38 \\f "Wingdings" \\s =
18}{\fldrslt\f14\fs36}}}{\b\f15\fs28 Heterologous cloning and =
expression of the xylanase gene from }{\b\i\f15\fs28 Chainia =
}{\b\f15\fs28=20
\par }\pard\plain =
\s5\qj\sb240\sa60\sl360\slmult1\nowidctlpar\outlinelevel4\adjustright =
\f1\fs22\lang2057 {\f15 The genomic library of }{\i\f15 Chainia }{\f15 =
in }{\f15 {\field{\*\fldinst SYMBOL 108 \\f "Symbol" \\s =
11}{\fldrslt\f3\fs22}}}{\f15  gt10 was analysed for the expression of =
the xylanase gene using antixylanase antibodies. The recombinants =
exhibiting positive enzyme linked immunosorbent assay test (ELISA) =
showed clearance on RBB-xylan plate. The phage lysates showed xylanase =
activity in the range of 19 to 36 mU ml}{\f15\super -1}{\f15 , which =
was higher than that exhibited by the previously cloned xylanase gene =
from }{\i\f15 Chainia}{\f15 . The pooled DNA preparation of EcoR1 =
digests of the xylanase-positive }{\f15 {\field{\*\fldinst SYMBOL 108 =
\\f "Symbol" \\s 11}{\fldrslt\f3\fs22}}}{\f15  gt10 recombinants =
showing higher activity was cloned in pUC8. One of the pUC8 =
recombinants (h}{\f15\sub 1}{\f15 ) which exhibited maximum activity =
(24 mU ml}{\f15\super -1}{\f15 ) was shown to code for the =
low-molecular weight xylanase from }{\i\f15 Chainia. }{\f15 The =
xylanase produced by h}{\f15\sub 1 }{\f15 was inducible by =
isopropylthio-}{\f15 {\field{\*\fldinst SYMBOL 98 \\f "Symbol" \\s =
11}{\fldrslt\f3\fs22}}}{\f15 -}{\scaps\f15 D}{\f15 -galactoside (IPTG) =
but not by xylan, suggesting that the DNA fragment containing the =
xylanase gene is in frame with, and its expression controlled by, the =
lacZ promoter.=20
\par }\pard\plain \qc\nowidctlpar\widctlpar\adjustright \fs20\cgrid {
\par=20
\par=20
\par=20
\par }\pard\plain \s17\qj\sa120\nowidctlpar\adjustright \fs20\lang2057 =
{\b\f15\fs36 {\field{\*\fldinst SYMBOL 38 \\f "Wingdings" \\s =
18}{\fldrslt\f14\fs36}}}{\b\f15\fs28 Enhancement of pH and =
thermostability of the xylanase}{\b\f15  }{\b\f15\fs28 from =
}{\b\i\f15\fs28 Chainia
\par }\pard\plain =
\s5\qj\sb240\sa60\sl360\slmult1\nowidctlpar\outlinelevel4\adjustright =
\f1\fs22\lang2057 {\f15 The ready accessibility of the xylanase II =
produced by }{\i\f15 Chainia}{\f15  to cellulose pulp due to its small =
size and the absence of cellulase are advantageous features. The enzyme =
is stable at 40}{\f15 {\field{\*\fldinst SYMBOL 176 \\f "Symbol" \\s =
11}{\fldrslt\f3\fs22}}}{\f15 C for 1h and in a pH range of 5-9 at =
4}{\f15 {\field{\*\fldinst SYMBOL 176 \\f "Symbol" \\s =
11}{\fldrslt\f3\fs22}}}{\f15 C. Improved stability of the enzyme at =
higher temperature and pH are desirable. Effect of a variety of =
compounds was studied to enhance stability. Glycerol, sorbitol, =
mannitol (10%) or glycine (1M) had marginal effect on thermostability. =
Addition of Ca }{\f15\super +2 }{\f15  or PEG (10mM) increased the =
half- life of the enzyme at 60}{\f15 {\field{\*\fldinst SYMBOL 176 \\f =
"Symbol" \\s 11}{\fldrslt\f3\fs22}}}{\f15 C. Cysteine (10mM) or =
Tween-80 (1%) showed 70% protection against thermal inactivation. Xylan =
(3%) offered complete protection against inactivation of the enzyme at =
60}{\f15 {\field{\*\fldinst SYMBOL 176 \\f "Symbol" \\s =
11}{\fldrslt\f3\fs22}}}{\f15 C and at pH 9.
\par }\pard\plain \nowidctlpar\widctlpar\adjustright \fs20\cgrid {
\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
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\par=20
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\par=20
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\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
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\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
\par=20
\par {\pntext\pard\plain\s5 \b\f14\fs28\cf1 =
\loch\af14\dbch\af0\hich\f14 \'d8\tab}}\pard\plain =
\s5\qj\fi-360\li360\sb240\sa60\nowidctlpar\jclisttab\tx360{\*\pn =
\pnlvlblt\ilvl0\ls11\pnrnot0\pnb1\pni0\pncf1\pnf14\pnfs28\pnstart1\pnind=
ent360\pnhang{\pntxtb \'d8}}\ls11\outlinelevel4\adjustright =
\f1\fs22\lang2057 {\b\f15\fs32 LIST OF PUBLICATIONS :
\par }\pard\plain \s18\nowidctlpar\widctlpar\adjustright \fs20\cgrid {
\par {\pntext\pard\plain\s16 \b\f15\fs22 \hich\af15\dbch\af0\loch\f15 =
1.\tab}}\pard\plain =
\s16\qj\fi-360\li360\sl360\slmult1\nowidctlpar\jclisttab\tx360{\*\pn =
\pnlvlbody\ilvl0\ls6\pnrnot0\pndec\pnb1\pni0\pnstart1\pnindent360\pnhang=
{\pntxta .}}\ls6\adjustright \fs20\lang2057 {\b\f15\fs22 K.R. =
Bandivadekar}{\f15\fs22  and V.V. Deshpande, (1994) \ldblquote Enhanced =
Stability of Cellulase-free Xylanase from }{\i\f15\fs22 =
Chainia\rdblquote }{\b\f15\fs22 , Biotech. Letters}{\f15\fs22  Vol. 16 =
No. 2, Page 179-182.}{\b\f15\fs22=20
\par {\pntext\pard\plain\s16 \b\f15\fs22 \hich\af15\dbch\af0\loch\f15 =
2.\tab}}\pard =
\s16\qj\fi-360\li360\sl360\slmult1\nowidctlpar\jclisttab\tx360{\*\pn =
\pnlvlbody\ilvl0\ls6\pnrnot0\pndec\pnb1\pni0\pnstart1\pnindent360\pnhang=
{\pntxta .}}\ls6\adjustright {\b\f15\fs22 K.R. Bandivadekar}{\f15\fs22  =
and V.V. Deshpande, (1996) \ldblquote Heterologous Cloning of Xylanase =
Gene from }{\i\f15\fs22 Chainia}{\f15\fs22 \rdblquote   }{\b\f15\fs22 =
Enzyme Microbial Technology}{\f15\fs22  Vol. 18:439-443}{\b\f15\fs22=20
\par {\pntext\pard\plain\s16 \b\f15\fs22 \hich\af15\dbch\af0\loch\f15 =
3.\tab}}\pard =
\s16\qj\fi-360\li360\sl360\slmult1\nowidctlpar\jclisttab\tx360{\*\pn =
\pnlvlbody\ilvl0\ls6\pnrnot0\pndec\pnb1\pni0\pnstart1\pnindent360\pnhang=
{\pntxta .}}\ls6\adjustright {\b\f15\fs22 K.R. Bandivadekar}{\f15\fs22  =
& V.V. Deshpande, (1996) \ldblquote Structure-function relationships of =
Xylanases : Fluorometric analysis of tryptophan environment\rdblquote , =
}{\b\f15\fs22 Biochem.  J}{\f15\fs22  Vol. 315, Pages =
583-587.}{\b\f15\fs22=20
\par {\pntext\pard\plain\s16 \b\f15\fs22 \hich\af15\dbch\af0\loch\f15 =
4.\tab}}\pard =
\s16\qj\fi-360\li360\sl360\slmult1\nowidctlpar\jclisttab\tx360{\*\pn =
\pnlvlbody\ilvl0\ls6\pnrnot0\pndec\pnb1\pni0\pnstart1\pnindent360\pnhang=
{\pntxta .}}\ls6\adjustright {\f15\fs22 M Rao, S Khadilkar, =
}{\b\f15\fs22 K.R. Bandivadekar}{\f15\fs22  & V.V. Deshpande, (1996) =
\ldblquote Structural Environment of an essential cysteine residue of =
xylanase from }{\i\f15\fs22 Chainia}{\f15\fs22  sp. (NCL =
82.5.1)\rdblquote , }{\b\f15\fs22 Biochem. J}{\f15\fs22  Vol. 316 pages =
771-775 .}{\b\f15\fs22=20
\par {\pntext\pard\plain\s16 \b\f15\fs22 \hich\af15\dbch\af0\loch\f15 =
5.\tab}}\pard =
\s16\qj\fi-360\li360\sl360\slmult1\nowidctlpar\jclisttab\tx360{\*\pn =
\pnlvlbody\ilvl0\ls6\pnrnot0\pndec\pnb1\pni0\pnstart1\pnindent360\pnhang=
{\pntxta .}}\ls6\adjustright {\b\f15\fs22 Kavita =
Bandivadekar}{\f15\fs22 , Suvarna Khadilkar, Mala Rao, Vasanti =
Deshpande (1997) \ldblquote Evidence of the essential carboxylate =
residue in the active site of xylanase from }{\i\f15\fs22 =
Chainia}{\f15\fs22  (NCL 82-5-1)\rdblquote , abstract accepted for the =
}{\b\f15\fs22 \ldblquote AMI Annual Conference\rdblquote }{\f15\fs22   =
held at New Delhi, India, during Dec 97.
\par {\pntext\pard\plain\b\v\fs22\lang1033\cgrid =
\hich\af0\dbch\af0\loch\f0 6.\tab}}\pard\plain =
\qj\fi-360\li360\sl360\slmult1\nowidctlpar\widctlpar\jclisttab\tx360{\*\=
pn =
\pnlvlbody\ilvl0\ls6\pnrnot0\pndec\pnb1\pni0\pnstart1\pnindent360\pnhang=
{\pntxta .}}\ls6\adjustright \fs20\cgrid {\b\v\fs22=20
\par }\pard \sl360\slmult1\nowidctlpar\widctlpar\adjustright {\fs22=20
\par }\pard \li1800\nowidctlpar\adjustright {\f15\fs22\cgrid0=20
\par }}
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