Chris Chilcott wrote:
>> Could someone please tell me how long I need to autocalve 5 litres of media
> for, or should I autocalve it in smaller volumes.
>> Regards
> Chris Chilcott
Hello, Chris!
This is from a laboratory manual:
Sterilizationtime 15 minutes at 121°C, 1 bar PLUS heatingtime!
Volume to be sterilized in ml Heatingtime in min
up to 50 5
50 - 100 8
100 - 500 12
500 - 1000 20
Out o fthis you can easily plot a regression curve and extrapolate to
the desired volume.
I hope this helps you.
Regards
Stefanie
