In situ bacterial DNA quantitation?

fuller at envirogen.com fuller at envirogen.com
Thu Oct 22 15:40:55 EST 1998

Dr. S. Shapiro wrote:
> Dear Colleagues;
>      I am curious if anyone is aware of a procedure for quantifying the
> DNA (or total nucleic acid) in individual bacterial cells that does not
> make use of radiosiotopes or flow cytometers.  For example, can it be done
> microscopically (electron microscopically) using particular fluorescent
> dyes?  Any other suggestions?
>      Kindly contact me directly at toukie at zui.unizh.ch.  Please provide
> reference citations.
> Thanks in advance to all responders,
> S. Shapiro
> toukie at zui.unizh.ch
> q

I'd suggest you contact Molecular Probes.  They have developed a large
variety of nucleic acid dyes, some specifically for quantification. 
Their web page is:


Hope this helps.


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