Dr. S. Shapiro wrote:
>> Dear Colleagues;
>> I am curious if anyone is aware of a procedure for quantifying the
> DNA (or total nucleic acid) in individual bacterial cells that does not
> make use of radiosiotopes or flow cytometers. For example, can it be done
> microscopically (electron microscopically) using particular fluorescent
> dyes? Any other suggestions?
>> Kindly contact me directly at toukie at zui.unizh.ch. Please provide
> reference citations.
>> Thanks in advance to all responders,
>> S. Shapiro
>toukie at zui.unizh.ch>> q
I'd suggest you contact Molecular Probes. They have developed a large
variety of nucleic acid dyes, some specifically for quantification.
Their web page is:
www.probes.com
Hope this helps.
-Mark
