HI
Trypan blue for testing for is, as I recall, a method that has been
around for quite a while. As a former biologist, I used trypan blue to
test the viability of white cells. Other stains can be used,such as
neutral red and acridine orange, though both have caveats about there
used. There was a great book called Methods in Cellular Immunology and
the author's name began with a M, who describes trypan blue as well as a
couple of other viability stains. A library search on supra-vital stains
should turn up a wealth of information for you. Good luck.
Michael
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On 16 Oct 1998, Mkh528 wrote:
> hello,
>> earlier in the week I saw a post regarding viability staining and someone
> recommended trying trypan blue from Sigma. after thinking about it I de-
> cided that it may be a useful idea to check out. I contacted Aldrich (of
> Sigma-Aldrich) and they had no knowledge of it being used for viability
> staining.
> the one reference i've heard about is its use in histology and its instruction.
> could whomever was involved in the postings please contact me and relate in
> what context the use of trypan blue was being discussed.
>> thanks in advance.
>> all the best,
>> michael
>michael.hall at robertmondavi.com>>
