Debra L. Swanson wrote:
>> Does anyone have a reliable protocol for cell staining that will differentiate
> viable from nonviable cells?
> Thanks.
>> Debra Swanson, Assistant Scientist
> Comparative Medicine
> University of Minnesota
> Minneapolis, MN 55455
>swans061 at maroon.tc.umn.edu
Dear Debra
The standard technique is to use trypan blue, available from Sigma. For
about 5-10 minutes after mixing (time depends on cell type) the viable
cells remain white or vaguely pink. dead cells turn blue. after a period
or time the dye does get into the live cells.
Mike
