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help me to prepare media for Pseudomonas

Robert Randolph rrrandolph at juno.com
Thu Nov 26 09:58:11 EST 1998

As a point of interest. Triclosan is Irgasan. It was a common addative
used by diaper services to kill all bacteria in the dirty laundry. It
was soon discovered that it did very well...except Ps. aeruginosa
survived. This was a big problem for them....a selective agent/medium
for the microbiologist.  R.Randolph
On 25 Nov 1998 12:07:53 -0800, ariel.finguerut at merconet.com.br ("Ariel
/ Jaime Finguerut") wrote:

>Hi, Bob:
>Do you have any idea about why there is Triclosan in this medium?
>As far as I know Triclosan is unsoluble in water.
>-----Mensagem original-----
>De: Bob Friedel <rfriedel at perrittlab.com>
>Para: microbio at net.bio.net <microbio at net.bio.net>
>Data: Terça-feira, 24 de Novembro de 1998 17:59
>Assunto: Re: help me to prepare media for Pseudomonas
>>Handbook of Microbiological Media
>>by Ron M. Atlas; Lawrence C. Parks (ed)
>>CRC Press, 1993, p.739
>>Isolation of Pseudomonas species:
>>Pseudomonas Isolation Agar
>>Composition per liter:
>>Peptone                         20.0 g
>>Agar                               13.6 g
>>K2SO4                           10.0 g
>>MgCl2-6H2O                   1.4 g
>>Triclosan                      0.025 g
>>Glycerol                          20.0 ml
>>Add components to distilled/deionized water and bring volume to 1.0 liter.
>>Mix thoroughly.  Gently heat and bring to boiling.  Distribute into tubes
>>flasks.  Autoclave for 15 minutes @ 15 PSI @ 121C.  Pour into sterile Petri
>>dishes or leave in tubes.
>>Final pH is 7.0 +/- 0.2
>>Available from BBL Microbiology Systems/Difco Laboratories USA
>>                          1-800-638-8663
>>Robert R. Friedel
>>(formerly Research Microbiologist, Whitehall-Robins Healthcare)
>>Quality Assurance Manager
>>Laboratory Research & Analysis Groups
>>Perritt Laboratories, Inc.
>>rfriedel at perrittlab.com
>>Edwin Pozharski wrote in message
>><365AC4AC.73CF2A9D at pbc.iteb.serpukhov.su>...
>>>Please, help me to find an information about how to prepare medium for
>>>Pseudomonas bacteriua. I will be appreciated for direct information on
>>>medium content or any kind of references.
>>>Thanks in advance.
>>>Lyubov Kolombet, PhD
>>>Institute of Applied Microbiology, Obolensk, Russia
>>>Email: kolomber at geocities.com

w_wallis at nose.it

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