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help me to prepare media for Pseudomonas

Ariel / Jaime Finguerut ariel.finguerut at merconet.com.br
Wed Nov 25 15:07:53 EST 1998

Hi, Bob:

Do you have any idea about why there is Triclosan in this medium?
As far as I know Triclosan is unsoluble in water.

-----Mensagem original-----
De: Bob Friedel <rfriedel at perrittlab.com>
Para: microbio at net.bio.net <microbio at net.bio.net>
Data: Terça-feira, 24 de Novembro de 1998 17:59
Assunto: Re: help me to prepare media for Pseudomonas

>Handbook of Microbiological Media
>by Ron M. Atlas; Lawrence C. Parks (ed)
>CRC Press, 1993, p.739
>Isolation of Pseudomonas species:
>Pseudomonas Isolation Agar
>Composition per liter:
>Peptone                         20.0 g
>Agar                               13.6 g
>K2SO4                           10.0 g
>MgCl2-6H2O                   1.4 g
>Triclosan                      0.025 g
>Glycerol                          20.0 ml
>Add components to distilled/deionized water and bring volume to 1.0 liter.
>Mix thoroughly.  Gently heat and bring to boiling.  Distribute into tubes
>flasks.  Autoclave for 15 minutes @ 15 PSI @ 121C.  Pour into sterile Petri
>dishes or leave in tubes.
>Final pH is 7.0 +/- 0.2
>Available from BBL Microbiology Systems/Difco Laboratories USA
>                          1-800-638-8663
>Robert R. Friedel
>(formerly Research Microbiologist, Whitehall-Robins Healthcare)
>Quality Assurance Manager
>Laboratory Research & Analysis Groups
>Perritt Laboratories, Inc.
>rfriedel at perrittlab.com
>Edwin Pozharski wrote in message
><365AC4AC.73CF2A9D at pbc.iteb.serpukhov.su>...
>>Please, help me to find an information about how to prepare medium for
>>Pseudomonas bacteriua. I will be appreciated for direct information on
>>medium content or any kind of references.
>>Thanks in advance.
>>Lyubov Kolombet, PhD
>>Institute of Applied Microbiology, Obolensk, Russia
>>Email: kolomber at geocities.com

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