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help me to prepare media for Pseudomonas

Yersinia yersinia at CYBERNEX.NET
Tue Nov 24 20:28:40 EST 1998

Hello, Rico and Lyubov,

Here is the recipe for  Cetrimide agar:

Per Liter:

Bacto Peptone:  20 g
Magnesium Chloride: 1.4 g
Potassium Sulfate:  10 g
Cetrimide (Cetyltrimethylammonium bromide):  0.3 g
Bacto Agar:  13.6 g

Or buy it from Difco.

Suspend 45.3 g of dehydrated Difco Centrimide medium in one liter 
distilled/deionized water.  Add 10 mL Bacto Glycerol and heat to boiling 
to dissolve.  Dispense in flasks or tubes.  Autoclave 15 min at 15 psi 
(or according to your autoclave's validation specs per volume).


>Subject:     Re: help me to prepare media for Pseudomonas
>Sent:        11/24/98 16:17
>Received:    11/24/98 19:42
>From:        Rico Coolen, ricoco at worldonline.nl
>To:          microbio at net.bio.net
>Hallo group / Lyobov,
>It depends on what purpose you have with culturing Pseudomonas spp.
>Nevertheless, I have worked with Cetrimide agar. I used it for enumeration
>of Ps. in meat and meat products.
>I have the recepy not available at this moment but if you wish I can search
>for it an send it to you by mail or by fax.
>Rico Coolen
>Quality assurence in food
>Van Rossem & Partners
>Edwin Pozharski heeft geschreven in bericht
><365AC4AC.73CF2A9D at pbc.iteb.serpukhov.su>...
>>Please, help me to find an information about how to prepare medium for
>>Pseudomonas bacteriua. I will be appreciated for direct information on
>>medium content or any kind of references.
>>Thanks in advance.
>>Lyubov Kolombet, PhD
>>Institute of Applied Microbiology, Obolensk, Russia
>>Email: kolomber at geocities.com

"A good microbiologist must always B. cereus."

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