Handbook of Microbiological Media
by Ron M. Atlas; Lawrence C. Parks (ed)
CRC Press, 1993, p.739
Isolation of Pseudomonas species:
Pseudomonas Isolation Agar
Composition per liter:
Peptone 20.0 g
Agar 13.6 g
K2SO4 10.0 g
MgCl2-6H2O 1.4 g
Triclosan 0.025 g
Glycerol 20.0 ml
Add components to distilled/deionized water and bring volume to 1.0 liter.
Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes of
flasks. Autoclave for 15 minutes @ 15 PSI @ 121C. Pour into sterile Petri
dishes or leave in tubes.
Final pH is 7.0 +/- 0.2
Available from BBL Microbiology Systems/Difco Laboratories USA
Robert R. Friedel
(formerly Research Microbiologist, Whitehall-Robins Healthcare)
Quality Assurance Manager
Laboratory Research & Analysis Groups
Perritt Laboratories, Inc.
rfriedel at perrittlab.com
Edwin Pozharski wrote in message
<365AC4AC.73CF2A9D at pbc.iteb.serpukhov.su>...
>Please, help me to find an information about how to prepare medium for
>Pseudomonas bacteriua. I will be appreciated for direct information on
>medium content or any kind of references.
>Thanks in advance.
>>Lyubov Kolombet, PhD
>Institute of Applied Microbiology, Obolensk, Russia
>Email: kolomber at geocities.com>