ying wrote:
>> i want to isolate staph and strep.
> Sample
> |
> Gram stain
> |
> Colombia nutrient agar
> |
> Catalase
> | |
> + ( -)
> Mannitol salt agar
> | |
> Heat tolerance
> Biochemical test
> Question
> 1. Will this method worked?
> 2. If catalase test is (-), can I confirm the sample contain strep?
> 3. Are there any possible contamination from other gram positive cocci?
> 4. Any other suggestion or comments?
> 5. Is it necessary to plate the sample on mannitol salt agar (if catalase
> test is positive) to select staph?
> 6. Can I substitute Colombia with any seletive media for both staph and
> strep?
The scheme seems confused. I'd 1st ensure a pure culture (use Columbia
blood agar) and note hemolysis. Then Gram stain, catalase, coagulase
and some of the API-type systems.
There are other catalse (-) bugs though your sample source may not offer
them.
