Hi All,
Can anyone tell me if there is any kind of relationship between
Klett units and Absorbance, please?
A protocol I am using calls for growing a broth culture to 50-70
Klett units, which is supposed to be about 10(8) cells per ml, or
mid-log phase. Unfortunately I only have a spec. that measures
absorbance values at a particular wavelength and I can't find
anything that describes Klett units.
Failing the relationship possibility, what would the absorbance of a
broth culture be at, say, 600nm wavelength at the mid-log phase (or
10(8)/ml).
To avoid annoying people, please reply direct to me at:
t.lyttle at botn.canterbury.ac.nz
Thanks for any help.
Trevor C. Lyttle
