Hi all,
I am at present trying to set up a transformation system for the gram
positive cutaneous organism Propionibacterium acnes. Previous work appeared
to show that conjugation and electroporation was unsuccessful, but as there
was no suitable selection marker, it was almost impossible to tell. In
addition, no endogenous P. acnes plasmids have been discovered thus far.
I have recently read a paper which details successful conjugation between E.
coli and Corynebacterium glutamicum (G+ve) by potentially knocking out the
host restriction system and think that this could be applied to
electroporation techniques. As such, I am now trying out different plasmids
and need help tracking a few down (from old papers, unable to find the
corresponding author etc.) and would appreciate any help, from kind authors
sending me the plasmids themselves (which would be wonderful), to the name
and address/email of someone who may be able to help. I have tried a number
of the vector databases on the web, and one plasmid at least is sold by ATCC
in America, but if possible, if only sold commercially, I would prefer a
European supplier (cheaper for me...grant money only goes so far, as my boss
keeps telling me!). The British NTCC has been little help, if only because a
number of these plasmids are extremely specialised (and I can't get onto
their website)
The list is; pJRD215 (G-ve broad host range cosmid)
pGK12 (source; Kok et al., Appl. Env. Micro., '84, 48, 726-731)
pECM1 (Schafer et al., J. Bact, '90, 172, 1663-1666)
pNG2 (Serwold-Davis et al. PNAS USA, '87, 84, 4964-4968)
and finally
Mobilisable E. coli strain S171
Thanks for any help you can give me
Yolie
