>>> If I have understood this properly, then you only get a part of the
> sequence. For identifying bacteria by 16S rDNA sequencing, a full
> blown
> sequence is required (or a least part of a full blown sequnce). G or T
>> ladders don't really help with identification, except perhaps for
> distinguishing between different strains of the same species.
>
You are indeed correct. There are sequence scanning methods out there
that by performing the G and T ladders onboth strands of a 16s RT-PCR
product, you essentially get sequence information. This of course is of
little use for direct ID of a biofilm organism, but the scanning method
greatly simplifies the tracing of the organism from apoint source to a
potential contamination site.
Fred
