I need advice on sample preparation of bacteria for scanning electron
microscopy. I have been growing p.denitrificans on carbon sem stubs as
biofilms. I would like to view how the bacteria colonize the stubs.
I have had one try where I simply dried the stubs at room temp prior to
observation. The images were poor and became charged very quickly.
Is coating of the specimen my only option?
Luke O'Brien
lob at eatl.co.uk
