IUBio

Listeria growth

Kevin Korth kkorth at cyberhighway.net
Tue Jun 2 21:09:52 EST 1998


V.K.Viswanathan wrote in message <6l1o1q$1cv at news.acns.nwu.edu>...
>I am trying to grow up some Listeria monocytogenes.  Could someone tell me
>what wavelength I should use to monitor the growth?  Thank you.


I assume you mean spectrophotometer?  I also assume you mean to do a growth
curve to estimate cfu/ml based on spec readings?

Try 550 to 600 nm.  As long as you set up your growth curve correctly, a
wavelength in that range should work just peachy.  Just stick with whatever
you started with.





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