IUBio

Pressure Cooker as Autoclave AGAIN

A.Ferszt a.ferszt at nospam.ic.ac.uk
Fri Jul 31 21:37:30 EST 1998


I see. Other than capped bottles, I've never sterilised the other items.
We do bag up Eppendorf-type tubes (in autoclave bags) and 'seal' them
after expelling as much air as we can, but I know the seal isn't truly
air-tight, as condensation sometimes appears in the bags.

How much simpler life would be if we had one of those nice autoclaves!


> 
> I did mean truly sealed, as per ampoules, capped bottles, Schucko bags etc
> but really used the wrong terminology in referring to boiling off.
> Probably exploding/imploding depending on the condition would be more
> appropriate.
> 
> A.Ferszt wrote
> > I don't think we're talking about exactly the same thing here. To me,
> > sealed means *really* sealed. There is no possibility of the liquid
> > contents boiling off, because they can't escape unless the bottle or cap
> > split (which certainly happens from time to time). That's why we always
> > back the screwcaps off a tiny bit. The concept of an autoclave that can
> > sterilise truly sealed bottles of liquid (and bottles that can take it)
> > is new to me. It doesn't surprise me that they would be found in
> > manufacturing facilities. Lucky people!
> 
> Ted Burrows.   Registered Authorised Person (Sterilizers)
> Integrated Designs
> Thermal Validation specialists



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