IUBio

Bacterial Susceptibility to Phage

Jay Mone' jmone at MARAUDER.MILLERSV.EDU
Wed Jul 29 10:11:05 EST 1998


Mariana,
There are at least two possible explanations that come to mind:
(1)  You are indeed seeing the appearance of bacteria resistant to the 
phage.  This is simple to demonstrate.  Do a plaque assay with a high 
enough phage titer to give complete lysis of the bacterial lawn.  Are 
there ant colonies of bacteria in the field of complete lysis?  if so, 
it indicates that the culture contains resistant cells.

(2)  Perhaps you are getting a large number of replication-defective 
phages, which lowers the overall pfu titer.  This is not uncommon with 
certain viruses, such as vesicular stomatitis virus.  As this virus is 
propageted in vitro, defective interfering particles are generated 
which powers virus titers.  This is a little more difficult to prove.

Jay Mone'



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