IUBio

Pressure Cooker as Autoclave AGAIN

Ted Burrows ted at integrated-designs.co.uk
Tue Jul 28 19:07:30 EST 1998


A.Ferszt wrote on Mon, 27 Jul 1998
> Good heavens! How do you keep the bottles from blowing up? We always
> tighten the cap, and then back it off a little. Once the load is cool,
> the caps are tightened before the bottles go on the shelf. The media
> never get contaminated. Sealed plastic bottles have been known to
> collapse as they cool.
> 
> I suppose the 'correct type of autoclave' is one that has never been in
> any of the university labs I've been in!

There are various ways of controlling the cooling stage so that the 
contents of sealed bottles do not boil off, ramped cooling, air 
ballasting, water shower and the same for plastic containers.

The sealing of the loose caps is common in media prep labs when quite 
often the media has to be taken out whilst still at the 80°C range.

Sealed fluids autoclaves are more normaly found in pharmaceutical 
production suites.

Ted Burrows.   Registered Authorised Person (Sterilizers)
Integrated Designs
Thermal Validation specialists




More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net