IUBio

bacterial growth curve vs. O.D.

Tobias Gaul Tobias.Gaul at t-online.de
Fri Jul 24 19:05:50 EST 1998


AFAIK and as far, as I have experienced: The cfu/OD-ratio depends on the
stage of growth of your culture. If you plate from a log-phase culture,
there will be more cfu's than from a stationary-phase culture.
There have been observations that suggest a high ratio of OD/Total cell
count to viable count. I don't know the source yet, but if you are
interested in, I will have a look.

Regards
TG

Franco Pagotto schrieb:
> 
> Hi all,
> 
> Is anyone aware of an equation which takes into account an OD reading
> and gives or estimates the CFU's/ml of a bacterial growth?
> 
> Tx.
> F.



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