IUBio

Pressure Cooker as Autoclave AGAIN

Ted Burrows ted at integrated-designs.co.uk
Wed Jul 22 20:59:29 EST 1998


Austin Reade wrote on Wed, 22 Jul 1998 15:50:23 GMT
> Good advice.  I am aware of a micro lab where staff were fortunate to 
> escape injury using a domestic pressure cooker to sterilise culture > 
media.

Be very careful if you sterilise fluid in sealed glass containers. 

Proper autoclaves for processing this type of load have built in safety 
controls that stop the doors being opened until the load is below 80°C, 
you don't have this with a pressure cooker.

The risk of the glass containers bursting when exposed to cold air is 
very real if the contents are above 80°C.

Ted Burrows.   Registered Authorised Person (Sterilizers)
Integrated Designs
Thermal Validation specialists




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