Suggestions: Peptide dyes for hydrophobic amino acids.

Glen Tamura gtamura at u.washington.edu
Fri Jul 10 17:42:52 EST 1998


How about transferring to a membrane and staining with India Ink? 


On Fri, 10 Jul 1998, Doug Stemke wrote:

> A graduate student in our department who is synthesizing a hydrophobic
> peptides has run into an interesting problem.  He has found that since he
> has no charged residues on his peptides he is unable to stain his peptides
> in SDS gels with either silver stain or Coomassie blue.  He has been doing
> some negative staining on his gels which work ok, but he is unable to
> effectively quantitate his results.  Does anyone here have a
> recommendation for a stain that would effectively bind hydrophobic amino
> acids in SDS gels?
> Thanks.
> Doug
> ******************************************************************
> Douglas J. Stemke, Ph.D.
> Biological Process Technology Institute
> University of Minnesota
> St. Paul, MN
> dstemke at biosci.cbs.umn.edu
> ******************************************************************

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