IUBio

enzyme activity

X. Zou webmaster at 101USA.ddpweb.com
Fri Jul 10 10:03:39 EST 1998


I am new to this newsgroup but it seemed like a good place to start.  I
am working with Beta 1,3 glucanase in seeds.  I have done an assay with
AZCL Pachyman and i have recorded absorbance at 590 nm.  Can anyone tell
me how to relate absorbance to the number of micromoles of reducing
sugar equivalents?  Also, can someone explain the Nelson/Somogyi
reducing sugar procedure?



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