A graduate student in our department who is synthesizing a hydrophobic
peptides has run into an interesting problem. He has found that since he
has no charged residues on his peptides he is unable to stain his peptides
in SDS gels with either silver stain or Coomassie blue. He has been doing
some negative staining on his gels which work ok, but he is unable to
effectively quantitate his results. Does anyone here have a
recommendation for a stain that would effectively bind hydrophobic amino
acids in SDS gels?
Thanks.
Doug
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Douglas J. Stemke, Ph.D.
Biological Process Technology Institute
University of Minnesota
St. Paul, MN
dstemke at biosci.cbs.umn.edu
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