Does anyone know of a high molecular weight protein marker for use with
SDS-PAGE that has a range around 100,000-500,00 Da?
I have tried Sigma's phosphorylase b cross-linked rabbit marker
(P8906) and followed the recommended protocol. I am only able to see
the 97,400 monomer; however, the dimer which should contain 28% overall
intensity, for lot#105H9400, is not visible.
Has anyone had luck with Sigma's phosphorylase b marker? If so,
which lot number was used and were any changes made to Sigma's
recommended protocol? For example, I have been unable to get the
majority of the protein into solution. Any suggestions?
Thanks for the help.
Marc Domanus
mhdoman at rs6000.cmp.ilstu.edu
