I have been trying unsuccessfully to perform PCR ribotyping on isolates
of Salmonella typhimurium and Pasteurella multocida by amplifying the
16S-23S intergenic spacer region. The primers I have been trying are
those used by Kostman et al (1992) J. Clin. Microbiol. 30:2084-2087 and
Nastasi et al (1997) J. Med. Micrbiol. 46:377-382. The sequences are as
5' - TTGTACACACCGCCCGTCA - 3'
5' - GGTACCTTAGATGTTTCAGTTC - 3'
So far, absolutely nothing has amplified, even when I follow the
published methods down to using the same brand of Taq. If anybody has
-any- ideas, please email me on kirsty.townsend at mailbox.uq.edu.au
Division of Veterinary Pathology and Anatomy
School of Veterinary Science and Animal Production
The University of Queensland
Brisbane 4072 AUSTRALIA
Ph: +61 7 3365 2667
Fax: +61 7 3365 1355