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PCR ribotyping with 16S-23S IGS

Kirsty Townsend kirsty.townsend at mailbox.uq.edu.au
Thu Jan 22 16:54:55 EST 1998

I have been trying unsuccessfully to perform PCR ribotyping on isolates
of Salmonella typhimurium and Pasteurella multocida by amplifying the
16S-23S intergenic spacer region. The primers I have been trying are
those used by Kostman et al (1992) J. Clin. Microbiol. 30:2084-2087 and
Nastasi et al (1997) J. Med. Micrbiol. 46:377-382. The sequences are as


So far, absolutely nothing has amplified, even when I follow the
published methods down to using the same brand of Taq. If anybody has
-any- ideas, please email me on kirsty.townsend at mailbox.uq.edu.au


Kirsty Townsend
Division of Veterinary Pathology and Anatomy
School of Veterinary Science and Animal Production
The University of Queensland
Brisbane 4072 AUSTRALIA

Ph: +61 7 3365 2667
Fax: +61 7 3365 1355

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