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Bio-rad assay

Thu Jan 15 14:13:48 EST 1998

Hi all,

I have just finished some growth studies on environmental bacteria 
which are capable of breaking down a certain chemical.  While I have 
recorded OD readings at 650nm for these bacteria I would also like to 
back these up by determining the amount of protein present using the 
above assay.

The samples after OD readings were centrifuged to pellet out the 
bacteria and allow the residual chemical to be analysed by UV.  So my 
samples are pelleted and currently frozen awaiting further analysis.
I would love to hear from people who have used this test (it seems 
straight forward enough) and would appreciate any advice available.  
Obviously I would have to thaw and re-centrifuge my samples before 

Thanks in advance for all replies.

              Kind regards,
                                     Maria Davoren

E-mail: MDavoren at athlone-rtc.ie

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