Frederick Venturi wrote:
>> What are Tn10d-Tc insertion mutations?
>
Tnd10d-Tc it is a mini-transposon, derived from Tn10. It has the
advantage that it does not has the transposase gene (d for deficient),
thus the insertions are very stable, and the insertion element does not
jump to other places in the genome creating new mutations, chromosome
reorganizations, etc. The Tc means resistance to tetracycline.
Probably, your version was created by Nancy Kleckner. There is a chapter
about it in the 'Bacterial genetics systems' volume of Methods in
Enzymology.
Tn10 is used broadly in gram-negative bacteria.
-Rafa
--
Rafael Maldonado
Division of Genetics
University of Alicante, Spain
rmaldonado at ua.es
