Markus Schneemann wrote:
> Hi all:
>> we want to purchase a quarz glass cuvette to measure DNA in a spec (100ul
> volume), and they are so outrageously expensive - $440/cuvette.
> Does anybody know a 'cheap' supplier ?
>> the same for TA cloning vectors - is there a reasonably priced vendor, or
> a recipe to make them by oneself ?
>> Cheers,
>> Markus
I don't know about the cuvettes, but one can use a vector that has tandem
XcmI sites which, when cut, will leave a T overhang analogous to that of the
TA kit (and it's much, much cheaper and bulkable).
I think the Cloning Vector Collection may have a few of these vectors. You
can find it at:
http://www.shigen.nig.ac.jp/cvector.html
They were very helpful in getting plasmids that I needed. It's very
economical (i.e. free!)
Good luck!!
CJ Fields
