Choice of enrichment for E.coli O157 ex food.

Damian Bond damian.bond at virgin.net
Tue Aug 11 12:48:34 EST 1998

CSL, a MAFF laboratory in the UK has completed a review of methods for O157
culture and isolation. They recommend mTSB for the enrichment, but CT-SMAC
for meats and CR-SMAC for milk. Again probably based upon levels of
commensurate flora. They also recommend 2 ims extraction stages - at 6 hours
and at 24 hours.

MTSB, CT-SMAC and IMS beads for O157 are all available commercially from LAB

john_m_nz at my-dejanews.com wrote in message
<6qmhdu$34h$1 at nnrp1.dejanews.com>...
>mTSB+n versus mEC+n as the preferred enrichment for culture or
>detection of O157 in MEAT.
>FDA BAM & AOAC describe mTSB+n (cefixime/vancomycin formula also
>is too inhibitory); USDA / FSIS use mEC+n.

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