Greetings. I have an idea, and I need advice. I would like to do
RT-PCR reactions on a specific cell type than is found in low frequency
in a larger cell population. We frequently isolate these cells by a
technique termed 'immunopanning' where we coat polystyrene with an
antibody directed against the cell type we want. Passing a mixture of
cells over the plate, only the desired cells adhere. For the RT-PCR,
our tubes are polypropylene. I do not know whether cells or antibody
protein would adhere to this surface. Has anyone had any experience
with this? Would coating with silane or something similar help? Thanks
for any replies. Please email me at twiemelt at wista.wistar.upenn.edu
Tony Wiemelt
The Wistar Institute
Philadelphia, PA
