Dear Colleagues:
I have been doing a little work with mice infected with fungi. I have
noticed that their serum samples, stored at minus 70 C have fungal growth at
the top. I haven't had time to pursue this, but this observation runs counter
to logic. Things don't grow on frozen media (though I once read that 15% of
the water is still in liquid phase) I think It more likely that there was
growth in the serum samples when I worked with these at room temperature, and
then upon freezing the fungi were somehow compressed to the top by the
formation of ice crystals. Any comment? Thanks...John
