Growth at minus 70 C

John Cherwonogrodzky jcherwon at dres.dnd.ca
Thu May 29 09:44:25 EST 1997

Dear Colleagues:
     I have been doing a little work with mice infected with fungi. I have 
noticed that their serum samples, stored at minus 70 C have fungal growth at 
the top. I haven't had time to pursue this, but this observation runs counter 
to logic. Things don't grow on frozen media (though I once read that 15% of 
the water is still in liquid phase) I think It more likely that there was 
growth in the serum samples when I worked with these at room temperature, and 
then upon freezing the fungi were somehow compressed to the top by the 
formation of ice crystals. Any comment? Thanks...John

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