I need to do immunocytochemical staining of a tissue culture plated on
top of a fibrin matrix. The problem is that my cells invade the matrix,
and they too need to be stained. I need to analyze them while they are
alive and in the matrix. (the best thing would be to extract them from
the matrix, but I can't do that)
can anyone suggest what to do? I was thinking of doing paraffin
embedding, but that will melt the matrix. The next best thing would be
a cryo-section.
Any suggestions are welcome
thanks
