help: immunocytochemistry problem

Martin Jugenburg martin.jugenburg at utoronto.ca
Tue May 27 20:17:49 EST 1997

I need to do immunocytochemical staining of a tissue culture plated on
top of a fibrin matrix.  The problem is that my cells invade the matrix,
and they too need to be stained.  I need to analyze them while they are
alive and in the matrix.  (the best thing would be to extract them from
the matrix, but I can't do that)

can anyone suggest what to do?  I was thinking of doing paraffin
embedding, but that will melt the matrix.  The next best thing would be
a cryo-section.

Any suggestions are welcome


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